منابع مشابه
Isolated Oxyntic Gland Preparation
Helicobacter pylon (H pyloon) infection is associated with hypo, normal, and hypersecretory disorders of the gastric mucosa. Pathophysiologicalpathways bywhichHpylori interacts with acid secretion are still unclear. The effects of H pylon on (14C) aminopyrine uptake by human parietal cells were examined as an indirect assay for acid secretion. Isolated oxyntic glands were stimulated with submax...
متن کاملThe isolated hepatocyte preparation: 30 years on.
A method for the preparation of intact rat hepatocytes in high yield was first described in 1969. The procedure involved digestion of hepatic tissue by perfusion of the liver with crude collagenase; later, purified collagenase without other enzymic additions was shown to be ineffective. Recently it has been discovered that the combination of purified collagenase plus elastase is superior to cru...
متن کاملPreparation of Isolated Rat Colonocytes
Suspensions of isolated colonic epithelial cells (colonocytes) have been obtained from rats by incubating everted lengths of colon with EDTA at 37°C in Krebs-Henseleit saline from which calcium was omitted and containing 0-25 % (w/v) bovine serum albumin. Measurements of oxygen consumption and lactate production by cell suspensions indicate that they are metabolically active for at least one ho...
متن کاملIsolated goat ileum preparation: An alternative to isolated ileum preparation from laboratory animals
Isolated tissue preparations are commonly used to study the effects of drugs on specific type of receptors. These preparations are also used for bioassay of drugs, characterisation of specific receptor or its subtypes, to determine concentration response curve of an agonist, to study antagonism of drug and in new drug discovery.[1] Isolated heart and rectus abdominis of frog are the two commonl...
متن کاملIsolated Goat Ileum Preparation – an Alternative to Isolated Ileum Preparation for the Three Point Bioassay of Acetylcholine
The objective of the study was to evaluate the use of goat ileum preparation for the estimation of unknown concentration of acetylcholine by three point bioassay. The graded doses of acetylcholine have shown dose dependent increase in contraction of isolated goat ileum. The responses obtained by this tissue preparation were similar to those with guinea pig ileum preparation. The goat ileum can ...
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ژورنال
عنوان ژورنال: British Journal of Anaesthesia
سال: 1979
ISSN: 0007-0912
DOI: 10.1093/bja/51.3.268-a